Temporary

Part:BBa_J70536:Design

Designed by: Julie Norville   Group: Knight Lab   (2009-12-08)


gfp RBS, {0,5;15,10} family member - B0033 simulator (forward oligo)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BioScaffold insert


Source

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;15,10})-GFP to (Promoter X)-(RBS level Y)-GFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)

(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)

Form of insert:

{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}

{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}

Forward oligo:

g {rbs master sequence} atg c gtaaa

Reverse oligo:

Reverse complement of (ctagag {rbs master sequence} atg c)

References